X-miR is a catalytic microRNA repressor more specific than antagonists.
The X-miR microRNA repressors target specific miRNA with match-driven catalysis and avoids the non-specific effects of typical microRNA antagonists. The X-miR can also be used in synthetic or stably expressed forms for research, drug-discovery, and therapeutics development- no chemical modifications required.
Mature microRNA's can be specifically repressed using Oligoengine's exclusive X-miR products. The X-miR is designed to bind to your target microRNA with perfect sequence complimentarity and avoid pitfalls associated with microRNA antagonists
Stable or Transient X-miR
Since chemical modifications or alternative chemistries are NOT required, the X-miR microRNA repressor can be constitutively expressed by inducible pX-miR or delivered as synthetic RNA.
Synthetic RNA X-miR: $499 each
Inducible pX-miR Clones: $599 each
Cloning oligos: $249 /oligo pair
X-miR Serum Stability
Transient use of synthetic X-miR RNA is prolonged by the exonuclease resistance characteristic of its novel structure.
Custom designed X-miRs
Thousands are pre-designed for specificity against individual miRNAs, but with new miRNAs discovered weekly, Oligoengine is ready to design X-miRs specific to your needs.
Getting started with X-miR microRNA repressors
X-miR oligos can be used transiently as RNA or expressed constitutively from inducible pX-miR vectors. Oligoengine offers design assistance for any custom X-miR as well as a library of pre-designed versions.
As non-coding regulatory elements, microRNA can be specifically repressed by X-miR oligo constructs.
Basic X-miR Design
Oligoengine designs X-miR specifically to a targeted nucleotides of a mature microRNA.
X-miR Design Overview
MicroRNA repression efficacy and duration is prolonged by exonuclease resistance.
Gene modulation results by microRNA repression will vary depending upon function of the microRNA itself. The X-miR libraries contains ready-to-use designs targeting 'mature', 'minor', pre-miRNA', or 'broad' nucleotide regions of each microRNA gene.
X-miR constructs are available as transfection-ready clones, ligation-ready oligos, synthetic RNA for transient use.
|pX-miR (Cloning oligo Set)||$249|
|Synthetic RNA X-miR (RNA, 20 nm)||$499|
Oligoengine can design your microRNA targeting X-miR. Design requests can be done via email or Fax-back forms.
Oligoengine 3.0 contains a listing of thousands of stock X-miR designs as well as tools for real-time design of your custom X-miR.
Any microRNA + 18 Days.
Pick any pX-miR, pSUPER or pSUPERIOR vector, a microRNA target, and we will send an X-miR clone for $499.
Cloning Service Deliverables:
• All clones are sequence-confirmed.
• 18 business day turnaround.
• Price includes all materials.
• Delivered as miniprep, ~4 µg.
Questions and Answers
Common Questions for X-miR.
Can I design my own X-miR?
> For best results, please request design and product quotes from Oligoengine via email, the fax forms, or Oligoengine software.
Will X-miR work with other vectors?
> Yes, we can provide ligation-ready X-miR oligos for your vector.
Is X-miR microRNA repression guaranteed?
> Yes, when the full suite of X-miRs for the targeted microRNA are purchased.
Can I use X-miR in-vivo?
> Yes, please Oligoengine contact customer service to optimize the design for your experiment.
Can I order my X-miR from another supplier?
> At the moment, X-miR microRNA repressors are exclusive to Oligoengine and its distributors. Licenses to other suppliers may be considered in the future.
Can I order X-miR oligos and clones internationally?
> Yes, Oligoengine will ship them to most countries or provide them through a distributor.
X-miR's are based on endoGUIDE technology in the AsymmetricRNAi Platform.
Academic and Non-Profit Licensing Terms
Researchers at academic and non-profit institutions are granted a limited end-user license with the purchase of each X-miR.
Commercial and For-Profit Licensing Terms
Use of the AsymmetricRNAi Platform, including X-miR, by commercial pharmaceutical and biotech companies is subject to specific licensing terms.
Companies can purchase individual X-miR molecules and clones before negotiating a streamlined product use agreement. Additionally, any products, including cell lines, transgenic animals and therapeutic compounds developed using X-miR will be considered as licensed products and subject to a sales royalty by Halo-Bio RNAi Therapeutics Inc.
For more information, please contact OligoEngine for details regarding research-to-therapeutics licensing support.