Empower your RNAi research with multi-gene suppression.
Multivalent RNA (MV-RNA) is a single RNAi molecule useful for targeting up to 3 seperate genes. A single MV-RNA can target one gene at several sites, or suppress multiple genes at once- depending upon the design. This novel design enables greater phenotype suppression potential than single siRNA, removes sense strand off-target elements, and replaces inaccurate ‘pooling’ methods of traditional siRNA.
Multivalent-RNA
MV-RNA are composed of three RNA guide strands 21-30nt long. Unlike siRNA, which contain a passenger and a guide strand, MV-RNA are composed of 3 guide strands- each aligning to a potential target site.
Single-Gene RNAi: Multi-site RISC
MV-RNA can be designed with guide strands to target divalent (2) or trivalent (3) sites on a single gene.
Multi-Gene RNAi: Single-site RISC
MV-RNA can be designed with three guide strands targeting different isoforms, genes in a pathway, or virus genes.
Synthetic MV-RNA
The MV-RNA reproduces the action of up to 3 siRNA duplexes to maximize potency while also minimizing irrelevant nucleic acids.
DICER SUBSTRATE MV-RNA
Some MV-RNA are designed for applications where a precursor MV-RNA are required for your research. MV-RNA can be stably expressed from your favorite vector, like pSUPER, or produced as ssRNA for transient experiments.
Uses in mammalian, animal, bioagriculture, and anthropod research are examples where 'Dicer-Substate MV-RNA' are advantageous.
Rnt1 SUBSTRATE MV-RNA
Rnt1 percursor MV-RNA are designed for applications where a Dicer enzymes may not be available. For example, some yeast do not have Dicer-like enzymes but utilize RNAi machinery.
Uses in fungal research, bioagriculture, and some mammalian research are examples where 'Rnt1-Substate MV-RNA' are potentially applicable.
Getting started with Multivalent RNA
Send us your target genes and Oligoengine will prepare designs of MV-RNA for you.
Design Overview of MV-RNA
MV-RNA strands are each designed to be complimentary to the target gene and also to specific regions of itself using Watson-Crick base pairing. The sequence composition supports the structural pattern by box-to-box relationship. The structured molecule is defined by 3 strands; PRIMARY, SECONDARY, and a KEY strand.
Design Overview of MV-RNA
Oligoengine designs MV-RNA specifically to a targeted regions of each gene.
Potential MV-RNA on Target Gene
Each target gene contains many potential sites that also satisfy complementarity for the structure of the MV-RNA.
MV-RNA Price List.
Synthetic MV-RNA, controls, and clones are available. Please see order form or contact Oligoengine
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Synthetic MV-RNA: | ||
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Synthetic MV-RNA (RNA, 20 nm) | $699 | |
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MV-RNA Clones: | ||
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MV-RNA Clones: | $699 | |
Custom MV-RNA Designs
Oligoengine can design your MV-RNA. Design requests can be done via email or Fax-back forms.
Stock MV-RNA
Oligoengine has stock MV-RNA as well as tools for real-time design of your custom MV-RNA.
MV-RNA Fax-Back Order Form
Use this fax form to place your MV-RNA order.
MV-RNA Clones:
Up to 3 Target Genes per MV-RNA + 18 Days.
Pick any pSUPER or pSUPERIOR vector, up to 3 target genes, and we will send an MV-RNA clone for $699.
Cloning Service Deliverables:
• All clones are sequence-confirmed.
• 18 business day turnaround.
• Price includes all materials.
• Delivered as miniprep, ~4 µg.
Cloning Fax Form.
Questions and Answers
Common Questions for MV-RNA.
Can I design my own MV-RNA?
> For best results, please request design and product quotes from Oligoengine via email, the fax forms, or Oligoengine software.
How do I convert an siRNA into a 'Multivalent RNA'?
> Please send your sequence and target gene(s) to Customer Service and we'll convert it to a univalent, divalent, or trivalent MV-RNA.
Will MV-RNA work with other vectors?
> Yes, we can provide ligation-ready MV-RNA oligos for your vector.
Is MV-RNA guaranteed?
> Yes, when two MV-RNA targeting the same genes are purchased.
Can I use MV-RNA in-vivo?
> Yes, please Oligoengine contact customer service to optimize the design for your experiment.
Can I order my MV-RNA from another supplier?
> At the moment, MV-RNA are exclusive to Oligoengine and its distributors. Licenses to other suppliers may be considered in the future.
Can I order MV-RNA oligos and clones internationally?
> Yes, Oligoengine will ship them to most countries or provide them through a distributor.
Are MV-RNA immunologically stimulating in mouse or human cells?
> MV-RNA are easily optimized for in-vivo use and exhibit a clean profile.
Can I order MV-RNA oligos and clones internationally?
> Yes, Oligoengine will ship them to most countries or provide them through a distributor.
Are MV-RNA functioning by RISC catalysis?
> Yes, the mode of action has been confirmed.
MV-RNA Licensing.
Oligoengine has expertise in multi-gene suppression and an elegant single-molecule solution.
If your product development in RNAi requires a pathway based approach, IP that is both novel and has a long life span, then we are happy to discuss our scalable license terms with you.
Academic and Non-Profit Licensing Terms
Researchers at academic and non-profit institutions are granted a limited end-user license with the purchase of each MV-RNA.
Commercial Research Use
MV-RNA are uniquely valuable for reverse genetics and pathway suppression studies at commercial institutions. Commercial Research licenses are obtainable from Halo-Bio RNAi Therapeutics, Inc.
Commercial Drug-discovery Use
MV-RNA for the discovery of vaccines, chemotherapies, and other pathway targeting drugs are available. Commercial Research licenses are obtainable from Halo-Bio RNAi Therapeutics, Inc.
Human Therapeutics Use
Licenses on a per-product basis are considered for partners seeking the unique properties of MV-RNA bases therapeutics. Commercial Research licenses are obtainable from Halo-Bio RNAi Therapeutics, Inc.
Bio-Agricultural Use
Please contact Halo-Bio RNAi Therapeutics, Inc.
Animal Therapeutics Use
Broad and specific licenses for animal therapeutics in broad geographies is considered. Please contact Halo-Bio RNAi Therapeutics, Inc.
Commercial and For-Profit Licensing Terms
Use of the AsymmetricRNAi Platform, including MV-RNA, by commercial pharmaceutical and biotech companies is subject to specific licensing terms.
Companies can purchase individual MV-RNA molecules and clones before negotiating a streamlined product use agreement. Additionally, any products, including cell lines, transgenic animals and therapeutic compounds developed using MV-RNA will be considered as licensed products and subject to a sales royalty by Halo-Bio RNAi Therapeutics Inc.
For more information, please contact OligoEngine for details regarding research-to-therapeutics licensing support.